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high capacity cdna reverse transcription kit (using random primers)  (Thermo Fisher)


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    Structured Review

    Thermo Fisher high capacity cdna reverse transcription kit (using random primers)
    High Capacity Cdna Reverse Transcription Kit (Using Random Primers), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+capacity+cdna+reverse+transcription+kit+(using+random+primers)/bio_rxiv__2024__12__01__626276-131-8-18
    Average 90 stars, based on 1 article reviews
    high capacity cdna reverse transcription kit (using random primers) - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Heterologous maternal antibodies derived from infectious bronchitis vaccines prevent the development of lesions associated with false layer syndrome.
    Article Snippet: First-strand cDNA synthesis was performed using random primers (High-Capacity Reverse Transcription KitTM, Applied Biosystems, Invitrogen Canada Inc., Burlington, ON, Canada), according to the manufacturer’s protocol.

    Article Title: Impact of a biostimulant enriched in betalain degradation products on ROS signaling, proline accumulation, and phytohormone homeostasis
    Article Snippet: Starting from 500 ng of total RNA, cDNA was synthesized for quantitative real-time polymerase chain reaction (qRT-PCR) analyses using random primers and the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA), following the manufacturer’s instructions [60]. qRT-PCR experiments were conducted on a QuantStudio 3 Real-Time PCR System (Applied Biosystems, Foster City, CA) using SYBR green I and ROX as an internal loading standard.

    Article Title: Enhancing immunotherapy through PD-L1 upregulation: the promising combination of anti-PD-L1 plus mTOR inhibitors.
    Article Snippet: Total RNA was extracted from cells, in basal conditions or after treatment using RNeasy kit (74104; Qiagen, Venlo, The Netherlands). cDNA was synthetized using random primers and High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). cDNA’s samples were amplified using specific primers and LightCycler 480 SYBR Green I Master (4707516001; Roche Diagnostics) in the QuantStudioTM 12K Flex (Applied BiosystemsTM).

    Article Title: A flexible, high-throughput system for studying live mRNA translation with HiBiT technology.
    Article Snippet: RNA was reverse transcribed using the High-Capacity cDNA Reverse Transcription kit (random primers) (Thermo Fisher Scientific, 4368814) according to the manufacturer’s instructions.

    Article Title: Modulation of regenerative responses by retinoic and ascorbic acids in human apical papilla cells.
    Article Snippet: Objective: This study investigated the bioactive effects of retinoic acid and ascorbic acid on hSCAPs in vitro.. Design: Cells were obtained from human third molars (n=4) and characterized for mesenchymal stem cell markers by flow cytometry.. The experimental groups: control (α-MEM); vehicle control group (α-MEM + 0.17 % DMSO); retinoic acid 0.1, 1, and 10 μM; and ascorbic acid 3, 30, and 300 μM (n=8) were tested for cell viability (alamarBlue; 1, 3, and 7 days), total collagen synthesis (Sirius Red; 1 and 7 days), mineralized matrix formation (Alizarin red; 14 days), and the regulation of gene expression related to mineralization (ALPL and DSPP), cell migration (ITGAV and CXCL12) angiogenesis (VEGFA) and collagen synthesis (COL1A1 and COL3A1; RT-qPCR) on 1 and 7 days.

    Article Title: Enhancing immunotherapy through PD ‐ L 1 upregulation: the promising combination of anti‐ PD ‐ L 1 plus m TOR inhibitors
    Article Snippet: Total RNA was extracted from cells, in basal conditions or after treatment using RNeasy kit (74104; Qiagen, Venlo, The Netherlands). cDNA was synthetized using random primers and High‐Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). cDNA's samples were amplified using specific primers and LightCycler® 480 SYBR Green I Master (4707516001; Roche Diagnostics) in the QuantStudioTM 12K Flex (Applied BiosystemsTM).

    Reverse Transcription:

    Article Title: Effects of Hypomethylating Agents on Gene Modulation in the Leukemic Microenvironment and Disease Trajectory in a Mouse Model of AML
    Article Snippet: Total RNA from whole blood was isolated using the Blood RNA Mini Kit (R02-01, Bioland Scientific; Paramount, CA, USA). .. Total RNA was converted to cDNA using the High Capacity cDNA Reverse Transcription Kit (using random primers) (4368814, Applied Biosystems; Waltham, MA, USA). cDNA was amplified and fluorescently labeled using the Power-UpTM 2x SYBRTM Green Mastermix for qPCR (containing ROX) (A25742, Applied Biosystems; Waltham, MA, USA). ..

    Article Title: NFAT activation by FKBP52 promotes cancer cell proliferation by suppressing p53
    Article Snippet: .. RNA was reverse-transcribed with random primers using High-Capacity cDNA Reverse Transcription Kit (4368814; ABI). qPCR was performed using FastStart Universal SYBR Green Master Mix (11226200; Roche) and StepOnePlus Real-Time PCR System (Applied Biosystems). ..

    Amplification:

    Article Title: Effects of Hypomethylating Agents on Gene Modulation in the Leukemic Microenvironment and Disease Trajectory in a Mouse Model of AML
    Article Snippet: Total RNA from whole blood was isolated using the Blood RNA Mini Kit (R02-01, Bioland Scientific; Paramount, CA, USA). .. Total RNA was converted to cDNA using the High Capacity cDNA Reverse Transcription Kit (using random primers) (4368814, Applied Biosystems; Waltham, MA, USA). cDNA was amplified and fluorescently labeled using the Power-UpTM 2x SYBRTM Green Mastermix for qPCR (containing ROX) (A25742, Applied Biosystems; Waltham, MA, USA). ..

    Labeling:

    Article Title: Effects of Hypomethylating Agents on Gene Modulation in the Leukemic Microenvironment and Disease Trajectory in a Mouse Model of AML
    Article Snippet: Total RNA from whole blood was isolated using the Blood RNA Mini Kit (R02-01, Bioland Scientific; Paramount, CA, USA). .. Total RNA was converted to cDNA using the High Capacity cDNA Reverse Transcription Kit (using random primers) (4368814, Applied Biosystems; Waltham, MA, USA). cDNA was amplified and fluorescently labeled using the Power-UpTM 2x SYBRTM Green Mastermix for qPCR (containing ROX) (A25742, Applied Biosystems; Waltham, MA, USA). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Effects of Hypomethylating Agents on Gene Modulation in the Leukemic Microenvironment and Disease Trajectory in a Mouse Model of AML
    Article Snippet: Total RNA from whole blood was isolated using the Blood RNA Mini Kit (R02-01, Bioland Scientific; Paramount, CA, USA). .. Total RNA was converted to cDNA using the High Capacity cDNA Reverse Transcription Kit (using random primers) (4368814, Applied Biosystems; Waltham, MA, USA). cDNA was amplified and fluorescently labeled using the Power-UpTM 2x SYBRTM Green Mastermix for qPCR (containing ROX) (A25742, Applied Biosystems; Waltham, MA, USA). ..

    Article Title: NFAT activation by FKBP52 promotes cancer cell proliferation by suppressing p53
    Article Snippet: .. RNA was reverse-transcribed with random primers using High-Capacity cDNA Reverse Transcription Kit (4368814; ABI). qPCR was performed using FastStart Universal SYBR Green Master Mix (11226200; Roche) and StepOnePlus Real-Time PCR System (Applied Biosystems). ..

    SYBR Green Assay:

    Article Title: NFAT activation by FKBP52 promotes cancer cell proliferation by suppressing p53
    Article Snippet: .. RNA was reverse-transcribed with random primers using High-Capacity cDNA Reverse Transcription Kit (4368814; ABI). qPCR was performed using FastStart Universal SYBR Green Master Mix (11226200; Roche) and StepOnePlus Real-Time PCR System (Applied Biosystems). ..



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    90
    Thermo Fisher high capacity cdna reverse transcription kit (using random primers)
    High Capacity Cdna Reverse Transcription Kit (Using Random Primers), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+capacity+cdna+reverse+transcription+kit+(using+random+primers)/bio_rxiv__2024__12__01__626276-131-8-18
    Average 90 stars, based on 1 article reviews
    high capacity cdna reverse transcription kit (using random primers) - by Bioz Stars, 2026-09
    90/100 stars
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